Coding

Part:BBa_K3747601

Designed by: Sanne van Doorn   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nir_operon

Nir_operon from Pseudomonas stutzeri JM300

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 11711
    Illegal EcoRI site found at 12635
    Illegal PstI site found at 391
    Illegal PstI site found at 469
    Illegal PstI site found at 7043
    Illegal PstI site found at 8069
    Illegal PstI site found at 8315
    Illegal PstI site found at 8894
    Illegal PstI site found at 9581
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 11711
    Illegal EcoRI site found at 12635
    Illegal NheI site found at 1779
    Illegal PstI site found at 391
    Illegal PstI site found at 469
    Illegal PstI site found at 7043
    Illegal PstI site found at 8069
    Illegal PstI site found at 8315
    Illegal PstI site found at 8894
    Illegal PstI site found at 9581
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 11711
    Illegal EcoRI site found at 12635
    Illegal BglII site found at 122
    Illegal BglII site found at 539
    Illegal BglII site found at 2595
    Illegal BglII site found at 5227
    Illegal BglII site found at 12759
    Illegal XhoI site found at 7796
    Illegal XhoI site found at 10694
    Illegal XhoI site found at 11933
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 11711
    Illegal EcoRI site found at 12635
    Illegal PstI site found at 391
    Illegal PstI site found at 469
    Illegal PstI site found at 7043
    Illegal PstI site found at 8069
    Illegal PstI site found at 8315
    Illegal PstI site found at 8894
    Illegal PstI site found at 9581
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 11711
    Illegal EcoRI site found at 12635
    Illegal PstI site found at 391
    Illegal PstI site found at 469
    Illegal PstI site found at 7043
    Illegal PstI site found at 8069
    Illegal PstI site found at 8315
    Illegal PstI site found at 8894
    Illegal PstI site found at 9581
    Illegal NgoMIV site found at 28
    Illegal NgoMIV site found at 40
    Illegal NgoMIV site found at 142
    Illegal NgoMIV site found at 502
    Illegal NgoMIV site found at 1303
    Illegal NgoMIV site found at 1801
    Illegal NgoMIV site found at 2764
    Illegal AgeI site found at 1090
    Illegal AgeI site found at 1636
    Illegal AgeI site found at 5166
    Illegal AgeI site found at 8206
  • 1000
    COMPATIBLE WITH RFC[1000]

The Nir operon consists of gene nirS, which is the catalytic subunit for the nitrite reductase. Included in the operon are multiple cytochromes and genes involved in heme d1 biosynthesis. These co-factors are required to complete the reduction of nitrite (NO2-) to nitric oxide (NO)[1]. Nir is the second enzyme in the denitrification pathway.


References

[1] Härtig, E., & Zumft, W. G. (1999). Kinetics of nirS expression (cytochrome cd 1 nitrite reductase) in Pseudomonas stutzeri during the transition from aerobic respiration to denitrification: evidence for a denitrification-specific nitrate-and nitrite-responsive regulatory system. Journal of Bacteriology, 181(1), 161-166.

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Categories
Parameters
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